The Erle Lab at the University of California San Francisco (UCSF) has immediate openings for postdoctoral fellows and a staff scientist interested in studying basic mechanisms underlying asthma. Asthma is a major public health problem that affects more than 300 million children and adults. Our studies, which are funded by the NIH’s National Heart Lung and Blood Institute (NHLBI) and National Institute for Allergy and Infectious Diseases (NIAID), focus on identifying how changes in the airway epithelium cause morbidity and mortality in asthma.
POSTDOCTORAL EXPERIMENTAL BIOLOGIST
This postdoctoral fellow will lead projects that make use of primary human bronchial epithelial cell culture models and other complementary systems to investigate gene regulation and mechanisms of airway obstruction in asthma. The fellow will build on prior studies from the lab that have identified a large set of transcriptional changes that occur in asthma and have begun to discover novel mechanisms that cause these changes and may be valuable therapeutic targets. The successful applicant will be encouraged to formulate innovative hypotheses and test them using methods already available in the lab augmented by other new methods as appropriate. Methods already in use in the lab include efficient gene targeting using CRISPR, microscopy-based methods for analyzing epithelial cell structure and function, single cell approaches to studying gene regulation (single cell RNA-seq, ATAC-seq, and flow cytometry), and massively parallel reporter assays. The fellow will work closely with other experienced lab members and collaborate with clinical researchers in other UCSF groups. Applicants should have experience with cell culture and molecular biology. Experience with immunofluorescence and/or video microscopy, flow cytometry, biochemistry, genomics, and basic computational biology and statistics would also be valuable but are not required; we expect to provide training in these areas as appropriate. Successful applicants will have a Ph.D. or equivalent, at least three years of relevant research experience, and excellent communication skills.
Applicants for the postdoctoral position should e-mail a CV and a one-page statement explaining their qualifications for and interest in a position to:
David J. Erle, MD
Professor and Associate Chair for Biomedical Research
Department of Medicine
University of California San Francisco
E-mail: [email protected]
STAFF RESEARCH ASSOCIATE II
The Erle lab at UCSF is seeking a Staff Research Associate (SRA) II interested in studying basic mechanisms underlying asthma. Asthma is a major public health problem that affects more than 300 million children and adults. Our studies, which are funded by the NIH’s National Heart Lung and Blood Institute (NHLBI) and National Institute for Allergy and Infectious Diseases (NIAID), focus on identifying how changes in the airway epithelium cause morbidity and mortality in asthma. We also collaborate with the Innovative Genomics Institute (IGI) at UC Berkeley on a Cystic Fibrosis Foundation (CFF)-funded grant assessing cell-specific delivery and editing of novel genome editing enzymes to correct orphan mutations in CF.
The SRA will join a team of scientists conducting experiments central to the lab’s research efforts in investigating gene regulation and mechanisms of airway obstruction in asthma and developing new treatment approaches for cystic fibrosis. This individual will contribute to research projects by generating datasets and testing hypotheses using methods established in the lab including primary human cell culture, flow cytometry, gene targeting using CRISPR-Cas9 and gene expression analysis. The SRA will work closely with other experienced lab members, collaborate with clinical researchers at UCSF, and have the opportunity to develop additional research skills and contribute to development of new research directions in the lab.
Successful applicants should have a BA/BS degree in a related scientific discipline and have 1+ years of related experience, or an equivalent combination of education and experience, plus excellent communication and organizational skills. Experience with mammalian cell culture is highly desirable. Proficiency in operation of standard cell biology laboratory equipment including microscopes, flow cytometers, and centrifuges is desirable.
The Erle lab is currently based at the Mission Bay campus but will be relocating to Parnassus mid-2020.
Applicants for the SRA II Position should apply through BrassRing.